Journal: Scandinavian journal of immunology

Article Title: Chitinase induce the release of IL-8 in human airway epithelial cells, via Ca2+-dependent PKC and ERK pathways.

doi: 10.1111/j.1365-3083.2010.02404.x

Figure Lengend Snippet: Figure 2 The effect of mitogen-activated protein kinase and NF-kB on Streptomyces griseus chitinase-induced IL-8 production. (A) Cells treated with chitinase were harvested at the indicated time points and then lysed. The equal amounts of cell extracts were resolved on 10% acryl- amide gels and then subjected to Western blot analysis. (B) Cells were pre-treated with indicated concentrations of U0126, SB202190 and JNK inhibitor II for 1 h prior to stimulation with 10 lg ⁄ ml S. griseus chitinase. The supernatants were collected and evaluated for IL-8 pro- duction by ELISA. **P £ 0.001 versus chitinase alone. (C) Cells were pre-treated with different concentrations of caffeine acid phenethyl ester (CAPE) for 1 h prior to stimulation with 10 lg ⁄ ml S. griseus chitinase. The supernatants were collected and evaluated for IL-8 production. *P £ 0.05 versus chitinase alone. The data represent the mean ± SEM of three separate experiments.

Article Snippet: Cells were plated at 2 · 105 cells ⁄ ml in 12-well culture plates and stimulated with chitinase (2 and 10 lg ⁄ ml) for 2, 6, 12 or 24 h. The supernatants were harvested and measured to determine the level of IL-8 by human IL-8 ELISA kit (R&D system, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Scandinavian journal of immunology

Article Title: Chitinase induce the release of IL-8 in human airway epithelial cells, via Ca2+-dependent PKC and ERK pathways.

doi: 10.1111/j.1365-3083.2010.02404.x

Figure Lengend Snippet: Figure 1 The effect of chitinase on IL-8 production and mRNA expres- sion. (A) Cells were stimulated with different concentrations of Strepto- myces griseus chitinase (2 and 10 lg ⁄ ml) at 37 C. The supernatants were collected and assayed for IL-8 by ELISA. **P < 0.001 versus con- trol alone, *P < 0.01 versus control alone. The data represent the mean ± SEM of four separate experiments. (B) The RNAs from cells treated with S. griseus chitinase were harvested and used for RT-PCR.

Article Snippet: Cells were plated at 2 · 105 cells ⁄ ml in 12-well culture plates and stimulated with chitinase (2 and 10 lg ⁄ ml) for 2, 6, 12 or 24 h. The supernatants were harvested and measured to determine the level of IL-8 by human IL-8 ELISA kit (R&D system, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Reverse Transcription Polymerase Chain Reaction

Journal: Scandinavian journal of immunology

Article Title: Chitinase induce the release of IL-8 in human airway epithelial cells, via Ca2+-dependent PKC and ERK pathways.

doi: 10.1111/j.1365-3083.2010.02404.x

Figure Lengend Snippet: Figure 5 The effect of Streptomyces griseus chitinase on IL-6 production. Cells were stimulated with different concentrations of S. griseus chitinase (2 and 10 lg ⁄ ml). The supernatants were collected and assayed for IL-8 by ELISA. **P £ 0.01 versus control alone. The data represent the mean ± SEM of four separate experiments.

Article Snippet: Cells were plated at 2 · 105 cells ⁄ ml in 12-well culture plates and stimulated with chitinase (2 and 10 lg ⁄ ml) for 2, 6, 12 or 24 h. The supernatants were harvested and measured to determine the level of IL-8 by human IL-8 ELISA kit (R&D system, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular Medicine

Article Title: Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent way

doi: 10.1186/s10020-021-00416-x

Figure Lengend Snippet: Metformin alleviates aortic calcification by activating the PI3K/AKT signaling pathway in a concentration-dependent manner in vitro. a Metformin inhibits inflammatory factor secretion including IL6, IL8, and MCP-1 in cell supernatant, after treatment with phosphate medium (PM) with or without metformin for 72 h as determined by ELISA; b ROS production was detected and quantified after PM treatment with or without metformin for 72 h; c Cell viability was detected by CCK8 after treatment with PM with or without metformin for 72 h; d Immunofluorescence staining images of p-AKT and OPN expression in AVICs after PM treatment with or without 100 μM metformin for 72 h, Scale bar, 50 μm; e The protein expression of p-AMPK (Thr172), p-AKT (Ser473), PI3K, p-eNOS (Ser1177), BMP2, and OPN in AVICs after PM treatment with or without metformin for 72 h as determined by WB; f Calcium deposition were detected by ARS Staining after various treatments for 7 days, Scale bar, 200 μm. n = 6 per group. CTL, control; Met, metformin; *p < 0.05 versus PM group (one-way ANOVA with Bonferroni post hoc test)

Article Snippet: The levels of IL6 (KE00007, Proteintech, China), IL8 (KE00006, Proteintech, China), and MCP-1 (KE00091, Proteintech, China) in the supernatant of AVICs were determined by ELISA.

Techniques: Concentration Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing, Control

Journal: Comprehensive Psychoneuroendocrinology

Article Title: Tone it down: Vagal nerve activity is associated with pro-inflammatory and anti-viral factors in breast cancer – An exploratory study

doi: 10.1016/j.cpnec.2021.100057

Figure Lengend Snippet: Cytokines introduced into the factor analysis and their correlations to each of the two factors.

Article Snippet: High-sensitivity IL-6 (HS600B), high-sensitivity IL-8 (HS800) and IL-18 (DY318), ELISA kits were purchased from R&D systems (Minneapolis, MN, USA); high-sensitivity IFNγ (BMS228HS) ELISA kits were purchased from eBioscience (San Diego, CA, USA).

Techniques:

Journal: Comprehensive Psychoneuroendocrinology

Article Title: Tone it down: Vagal nerve activity is associated with pro-inflammatory and anti-viral factors in breast cancer – An exploratory study

doi: 10.1016/j.cpnec.2021.100057

Figure Lengend Snippet: SDNN values and Cytokine serum levels (pg/ml).

Article Snippet: High-sensitivity IL-6 (HS600B), high-sensitivity IL-8 (HS800) and IL-18 (DY318), ELISA kits were purchased from R&D systems (Minneapolis, MN, USA); high-sensitivity IFNγ (BMS228HS) ELISA kits were purchased from eBioscience (San Diego, CA, USA).

Techniques: